Coding

Part:BBa_K1973002:Design

Designed by: Laura Claret Fernndez   Group: iGEM16_UPO-Sevilla   (2016-10-09)


xylS2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 50
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 772
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 235


Design Notes

We have inlcuded the sequence of RBS BBa_B0034 by means of the PCR primers in the amplification, as well as the scar that should have been when clonning this part.


Source

It was obtained by PCR from a plasmid containing its sequence.

References